What experiments or research methods can be used to assess the effect of bottle color on the shelf life of health supplements?

Accelerated aging test
Principle: By simulating the storage conditions for a long time, the quality change of health care products is observed in a short time. The experiment takes advantage of the principle of chemical dynamics, which means that an increase in temperature, light and other factors will accelerate the speed of chemical reactions.
Steps:
Prepare multiple sets of the same health care product samples, each into different colors (such as clear, light blue, brown, black, etc.) bottles.
These samples are placed in a temperature-controlled, light-controlled environment chamber with a high temperature (e.g., 40-50 ° C) and a strong light intensity (simulating direct sunlight or strong light exposure environment).
At different time intervals (such as 1 week, 2 weeks, 4 weeks, etc.), samples were taken from the environmental tank to detect key indicators such as active ingredient content and activity of health care products. The content of active components can be determined by chemical analysis methods, such as high performance liquid chromatography (HPLC) to determine the content of vitamins, minerals and other components; For health products containing bioactive ingredients (such as enzymes, probiotics), the status of the active ingredient can be assessed by specific biological testing methods, such as enzyme activity determination, colony counting, etc.
The effect of bottle color on shelf life was evaluated by comparing the rate of decline of key indicators of health care products in different colored bottles. For example, if the vitamin C content in brown bottles decreased by 10% after 4 weeks of accelerated aging experiment, but decreased by 30% in clear bottles, it can be preliminarily determined that brown bottles are more favorable for vitamin C preservation, possibly extending shelf life.
Light stability experiment
Principle: Focus on the impact of light on health care products, because different color bottles have different transmittance and absorption rates of light. The energy of light can excite molecules in health supplements, leading to chemical reactions that can affect shelf life.
Steps:
Also prepare multiple groups of the same health care product samples and put them into different colored bottles.
These bottles are placed in a light box, which can produce light of different wavelengths (such as ultraviolet, visible light, etc.), and can control the intensity and time of the light.
For each group of samples, different lighting conditions are set, such as ultraviolet light only, visible light only or full spectrum irradiation, and the irradiation time is controlled (such as 24 hours, 48 hours, 72 hours, etc.).
After the end of irradiation, the composition and properties of the health care products are tested in a manner similar to the accelerated aging experiment, including chemical analysis and biological detection. The influence of color on light stability was determined by comparing the variation degree of the ingredients of health products in different color bottles under the same lighting conditions. For example, the active ingredient retention rate of CoQ10 samples in black bottles was higher than that in clear bottles when exposed to UV light, suggesting that black bottles are more effective at preventing UV damage to CoQ10 and may help extend shelf life.
Long-term storage experiment
Principle: Under normal storage conditions, after a long time of observation, the most real bottle color on the shelf life of health products to obtain the data. This approach is closer to the actual consumption and storage situation.
Steps:
Select the same health care product packaged in a variety of different color bottles to ensure the same initial product quality (including ingredients, production date, etc.).
Place these products in a regular storage environment, such as a warehouse or home medicine cabinet, and control the ambient temperature within the normal range (e.g. 15-25 ° C), away from direct sunlight.
Regular (such as every 3 months, 6 months, 12 months, etc.) sampling testing of health care products, including the content of active ingredients, appearance changes (such as whether there is precipitation, discoloration, etc.), odor changes, etc. Through long-term tracking and testing, record the quality problems (such as the content of active ingredients below the standard, odor, etc.) of health care products in different color bottles, so as to evaluate the impact of bottle color on the shelf life. For example, if a supplement packaged in a brown bottle still meets the standard active ingredient content after 36 months, while the same product packaged in a clear bottle has insufficient active ingredient content after 30 months, then the brown bottle can be considered to have a positive impact on the shelf life of the supplement.
Microbial Challenge experiment (for health products containing microbial ingredients such as probiotics)
Principle: For health products containing microbial ingredients, the survival of microorganisms directly affects product quality and shelf life. The effect of different colored bottles on light and temperature changes the environment for microorganisms.
Steps:
Prepare health supplements containing microbial ingredients such as probiotics and fill them in different colored bottles.
These bottles are placed under different environmental conditions, including environments with different light intensities and environments with different temperatures (such as simulating high summer temperatures and low winter temperatures).
After a certain interval (such as 1 week, 2 weeks, etc.), microbial detection methods, such as plate counting method, are used to count the number of microorganisms in the bottle. The survival quantity and activity of microorganisms in different colored bottles were compared to determine the influence of bottle color on microbial survival, and then the influence on the shelf life of health products containing microbial ingredients was evaluated. For example, in the high temperature and light environment, the survival number of probiotics in dark bottles was found to be significantly higher than that in transparent bottles, which indicates that dark bottles provide a more suitable living environment for probiotics, which is conducive to extending the shelf life of such health products